@Bplatinum9 I have been meaning to post this. If you are using something like H2O2, like ZeroTol HC, it will need a minimum of 5 minutes contact time to kill off bacteria. It kills mold and mildew spores in 1-2 minutes. Not sure if you do this already, don’t have time right now to skim through the recent posts, but what I have been doing is filling my reservoir with 3 gallons of tap water and mixing in the ZeroTol HC. I then turn on the pump and circulate it through the entire system. Bit of a pain in the ass running the feed hoses from the bubbler back to the reservoir, but I have found that this does an excellent job at cleaning everything out.
During my last major cleaning of my tent, I circulated ZeroTol HC and Hygrozyme through everything for a couple hours. The Hygrozyme helps break up any gunk that is stuck to the sides of your feeding tubes, along with doing some maintenance inside the bubbler. It was pretty amazing how much stuff the Hygrozyme was able to clear out of my system, there was chunks of stuff floating around in my reservoir when I drained it.