Is this caused by Bottle 5 or is it root rot?

The plant is in otherwise good shape

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Its bacteria growth. I have it every grow. My unit is two years old and some of that has permanently stained parts of the unit. I suspect some isopropyl and hard scrubbing would get it off. Just make sure both sides of the lid are clean when your cleaning. Soak in vinegar water to clean

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also check where hose 5 is dispensing

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I assume I would be doing this after the end of my plant cycle?

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Yup. If it builds up bad you can wipe it off gently at early stages. Later in growth you wont be able too

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Thank you so much!!! The tube to bottle 5 crept up so I readjusted it to below the solution. Can’t thank you enough

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I would think you would have brown spots on leaves if #5 wasn’t dispensing

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